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M94A3324.TXT
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1994-10-25
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Document 3324
DOCN M94A3324
TI Unessential of calcium ions and inhibitor specificity for the
intracellular gp160 processing.
DT 9412
AU Okumura Y; Kamoshita K; Koga Y; Sasaki M; Kido H; Inst. for Enz. Res.,
Univ. of Tokushima, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):101 (abstract no. PA0024). Unique
Identifier : AIDSLINE ICA10/94369251
AB OBJECTIVES: Endoproteolytic cleavage of the envelope glycoprotein
precursor (gp160) of HIV-1 by cellular protease(s) is required for the
full activity of virus. In this study, the requirement of calcium ions
and the inhibitor specificity for the intracellular gp160 processing
were analyzed. METHOD: Intracellular gp160 processing was analyzed in
cultured HeLa cells and Molt-4, clone 8 cells which infected with
recombinant vaccinia virus encoded gp160 gene. RESULTS AND CONCLUSIONS:
Intracellular calcium ions depletion by 10(-6)M of EGTA, or EDTA or by
10(-7)M of calcium-ionophore, A23187 in the calcium free medium and
intracellular calcium ions administration by incubation with A23187 and
2mM of CaCl2 had no effect on the gp160 processing in cultured HeLa
cells and Molt-4, clone 8 cells. The gp160 processing in these cells was
inhibited by substrate analog dec-RVKR-cmk, thiol reagents, heavy metal
ions and low molecular weight serine protease inhibitor diisopropyl
fluorophosphate. The results suggest that intracellular gp160 processing
enzyme in these cells is not a member of Furin-like proteases and that
the processing protease has serine and cysteine residues in the active
site region.
DE Amino Acid Sequence Binding Sites Calcimycin/PHARMACOLOGY
Calcium/*METABOLISM Cell Line Edetic Acid/PHARMACOLOGY Egtazic
Acid/PHARMACOLOGY Gene Products, env/GENETICS/*METABOLISM Hela Cells
Human HIV-1/DRUG EFFECTS/*METABOLISM Molecular Sequence Data
Oligopeptides/CHEMISTRY/PHARMACOLOGY Peptide
Peptidohydrolases/METABOLISM Protein Precursors/GENETICS/*METABOLISM
Protein Processing, Post-Translational/DRUG EFFECTS Recombinant
Proteins/GENETICS/METABOLISM MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).